F4100-03M
Fetoprotein, Alpha (Alpha Fetoprotein, Alpha, Alpha-fetoprotein Precursor, AFP, Alpha-1-fetoprotein, Alpha-fetoglobulin, FETA, HPAFP) (FITC)
Größe
£594.00
- SKU:
- F4100-03M
- Anwendung:
- FLISA
- Reinheit:
- Purified by DEAE chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Purified human alpha-foetoprotein is isolated from pooled amniotic fluid and hepatoma serum
- Format:
- Supplied as a lyophilized powder in PBS, pH 7.2. Preservative free. Reconstitute with 1ml sterile dH2O.
- Weitere Details:
- One of the best studied is the alpha-foetoprotein. It cab easily be identified immunochemically because of its distinct antigenicity. In electrophoresis its relative mobility is between the albumin and alpha-1 globulin fractions. Its molecular weight is 64000, the carbohydrate content is relatively low. The concentration of alpha-foetoprotein gradually decreases during foetal growth. It is not found in normal adult serum, but reappears in most human developing hepatoma. Applications: Suitable for use in FLISA, Immunoelectrophoresis and Single and Double Radial Immunodiffusion. Other applications not tested. Recommended Dilution: Immunoelectrophoresis: 2ml plasma or equivalent against 120ml antiserum Double Radial Immunodiffusion: Use a rosette arrangement with 10ml antiserum in 3mm diameter center well and 2ml plasma samples (neat and serially diluted) in 2mm diameter peripheral wells. Single Radial Immunodiffusion: 1% in the gel. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice