F4100-01K
Fetoprotein, Alpha (Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP) [KO Validated]
Größe
£895.00
- SKU:
- F4100-01K
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human umbilical cord serum-derived alpha-Fetoprotein
- Klon:
- 9H119
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- AFP (alpha-Fetoprotein) is a 69-73 kDa member of the ALB/AFP/VDB family of proteins. Alpha-Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids. Mature human AFP is 591 amino acids (aa) in length. It contains three albumin domains (aa 19-210, 211-402 and 403-601), plus 15 intrachain disulfide bonds. Mature human AFP shares approximately 66% aa identity with mouse AFP. Applications: Suitable for use in Direct ELISA, Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 8-25ug/ml using Immersion fixed HepG2 human hepatocellular carcinoma cell line Flow Cytometry: 2.5ug/10e6 cells using HepG2 human hepatocellular carcinoma cell line fixed with paraformalydehyde and permeabilized with saponin. KO Validated: alpha -Fetoprotein/AFP was specifically detected in immersion fixed HepG2 human hepatocellular carcinoma cell line but is not detected in alpha -Fetoprotein/AFP knockout (KO) HepG2 cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice