F0025-79B
FcRL6 (Fc Receptor-like Protein 6, FcR-like Protein 6, Fc Receptor Homolog 6, IFGP6, FCRH6)
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£825.00
- SKU:
- F0025-79B
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein A/G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to aa16-312 from human FCRL6 expressed in CHO cell line.
- Klon:
- 12K96
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- FCRL6 (Fc receptor-like protein 6; also FcRH6 and IFGP6) is a 66kD member of the Ig Superfamily. It is found on CD56dim CD16+ NK cells, CD56+ CD3+ NKT cells, gd T cells, and effector plus effector-memory CD8+ T cells. FCRL6 is not a receptor for immunoglobulin. It does, however, bind to isoforms of HLA-DR that are expressed on APCs. Given that FCRL6 contains a cytoplasmic ITIM, FCRL6 may function as an inhibitory receptor for MHC Class II. Mature human FCRL6 is a 415aa type I transmembrane protein. It contains a 288aa extracellular region aa20-307 that shows three C2-type Ig-like domains aa20-293, and a 106aa cytoplasmic domain. There are multiple potential splice variants. One shows a deletion of aa105-200 accompanied by a 13aa substitution for aa394-434; a second possesses a 15aa substitution for aa383-434; and a third shows an alternative start site seven aa upstream of the standard site accompanied by a 13aa substitution for aa394-434. Over aa16-312, human FCRL6 shares only 33% aa identity with the mouse counterpart to FCRL6. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug labels 10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice