F0019-91
Fatty Acid Binding Protein 8 (FABP8, M [myelin]FABP, P2, PMP2)
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£754.00
- SKU:
- F0019-91
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to Ser2-Val132 from human FABP8/M-FABP expressed in E. coli.
- Klon:
- 11n70
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- FABP8 is a 15kD (predicted) member of the fatty acid binding protein family, calycin superfamily of molecules. It is found in Schwann cells, presumably on the cytoplasmic face of the plasma membrane where it may contribute to fatty acid transport across myelin. Functionally, FABP8 has a high affinity for U-shaped fatty acids such as oleic and palmitic acid. Human FABP8 is 132aa in length and exhibits two layers of antiparallel b-strands that envelope a hydrophobic pocket for lipid binding. Arg107 plus Arg127-Ile128-Tyr129 participate in fatty acid binding. Full length human FABP8 shares 87-95aa identity with mouse and rabbit FABP9, respectively. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml using human brain (cerebellum) tissue Immunohistochemistry: 8-25ug/ml in immersion fixed paraffin-embedded sections of hujman spinal cord Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice