Fas (Tumor Necrosis Factor Receptor Superfamily Member 6, Apo-1 Antigen, Apoptosis-mediating Surface Antigen FAS, FASLG Receptor, CD95, APT1, FAS1, TNFRSF6)

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£512.00

SKU:: F0019-63A
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to a sequence at the N-terminal of rat FAS, different from the related mouse sequence by 7aa.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.
Weitere Details:: Fas/APO-1/CD95 is a 36kD cell surface type I-membrane glycoprotein with an apparent molecular weight of 44kD on SDS-PAGE. Fas is a member of the tumor necrosis factor receptor (TNFR) family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis of Fas expressing cells. Both Fas and TNFR contain a 70aa cytoplasmic death domain that is responsible for transmitting signal for apoptosis. Apoptosis induced by Fas-Fas ligand interaction is inhibited by a number of cytosolic factors such as the ICE/CED3 family inhibitor CrmA, and the short and long form of FLIP. Insertion of an early transposable element in intron 2 of Fas structural gene, as in the mouse lpr mutations, causes lymphoproliferation and autoimmunity in mutant mice, suggesting that Fas is involved in clonal deletion of autoreactive T cells. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry, and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunohistochemistry (frozen/paraffin): 1-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice