F0019-50F

Factor XIIIa (Fibrinoligase, Plasma Transglutaminase, Fibrin Stabilizing Factor)

Größe

£953.00

SKU:: F0019-50F
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to a-subunit of coagulation Factor XIII. Cellular Localization: Cytoplasmic and nuclear. MW of Antigen: 160kD (unreduced dimmer); 80kD (reduced monomer).
Klon:: 3F177
Format:: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See F0019-50FX.
Weitere Details:: Factor XIII is a b-globulin found in plasma and is composed of two subunits. Factor XIIIa is the catalytic subunit and is a dimer of MW 160kD. Factor XIII is present in plasma as an a2b2 heterodimer (M.W. 320kD); whereas in platelets, only the a2 unit exists. Factor XIIIa is a dermal dendrocyte marker and shows variable reaction with these types of tumors. It can be used for histiocytic phenotyping and has been reported to mark capillary hemangiomas and tumors of the central nervous system. Factor XIII has also been used with CD34 to differentiate between dermatofibroma and dermatofibrosarcoma protuberans. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1:100 for 20 minutes at RT. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues. Optimal dilutions to be determined by the researcher. Positive Control: Recombinant human Factor XIII a-subunit, capillary hemangioma, dermatofibroma, and placenta. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice