F0015-01C
Factor VII (Coagulation Factor VII, F7, Eptacog alfa, Proconvertin, Serum Prothrombin Conversion Accelerator, SPCA) (Not for Export EU)
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£704.00
- SKU:
- F0015-01C
- Reinheit:
- Serum
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Purified from plasma.
- Format:
- Supplied as a lyophilized powder in PBS, pH 7.2. Preservative free. Reconstitute with 1ml sterile dH2O.
- Weitere Details:
- Coagulation factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF) and is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and also has important coagulation-independent functions such as angiogenesis. The predominant splicing variant of Factor VII in normal liver corresponds to the 444aa precursor. After cleavage of a signal peptide (residues 1-38), the mature chain can be further processed into the light chain (residues 39-190) and the heavy chain (residues 191-444). Applications: Suitable for use in ELISA, Immunoelectrophoresis and single and double radial immunodiffusion. Other applications not tested. Recommended Dilution: Immunoelectrophoresis: 2ml plasma or equivalent against 120ml antiserum Double Radial Immunodiffusion: Use a rosette arrangement with 10ml antiserum in 3mm diameter center well and 2ml plasma samples (neat and serially diluted) in 2mm diameter peripheral wells. Single Radial Immunodiffusion: 1% in the gel. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice