E9100-27-ML650
Ezrin (Radixin, Moesin, Cytovillin, p81, Membrane-organizing extension spike protein) (MaxLight 650)
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£991.00
- SKU:
- E9100-27-ML650
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein containing the C-terminal sequence of human ezrin
- Klon:
- 3C12
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
- Weitere Details:
- MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Ezrin, a member of the ERM (ezrin/radixin/moesin) family of proteins, is a ~80kD mediator of the interaction between actin filaments and the plasma membrane. In its interaction with the cytoskeleton, ezrin is involved in the formation of microvilli, cell-cell adhesion, cell shape, motility, membrane trafficking, and signal transduction. Ezrin is composed of a C-terminal actin-binding domain, an alpha-helical region, and an N-terminal domain that interacts with CD43, CD44, ICAM-1, ICAM-2, and PIP2 (phosphatidylinositol 4,5-biphosphate). Ezrin’s function is regulated by its conformation; the amino- and carboxy-termini associate head-to-tail, blocking the F-actin binding domain in ezrin’s native conformation. Merlin, the protein product of the tumor suppressor gene neurofibromatosis-2 (NF2), also binds to ezrin in this head-to-tail orientation. Phosphorylation of threonine and tyrosine residues has been implicated in ezrin protein activity. Ezrin phosphorylation has been reported on tyrosine residues after growth factor stimulation, and the phosphorylation of a conserved threonine (T567) is recognized as a key regulator in the transition from membrane-bound ezrin oligomers to active monomers, which induce the formation of actin-rich membrane projections. Because of its role in cell migration and recognition by the immune system, ezrin expression has been studied in a variety of normal and malignant tissues and cell lines. Ezrin is localized intracellularly under the plasma membrane in microvilli and at sites of cell-cell contact; normal tissues with high levels of expression include intestine, kidney, and placenta. In cell lines, ezrin expression has been observed in endometrial, pancreatic, colorectal, and breast carcinomas, and tissue expression has been described in uveal melanoma, the stromal cells of hemangioblastoma (but not glioblastoma), and astrocytoma (but not oligodendroglioma). Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation, Immunofluorescence and Western Blot. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (paraffin): 1–5ug/ml Immunoprecipitation: 1-5ug/ml Immunofluorescence: 1–5ug/ml Western Blot: 0.5–2ug/ml Note: Immunohistochemistry assays with formalin-fixed, paraffin-embedded tissue sections require HIER (heat induced epitope retrieval) with citrate buffer, pH 6.0. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice