E8949-40M
EIF4E, NT (Eukaryotic Translation Initiation Factor 4E, eIF4E, eIF-4E, mRNA Cap-binding Protein, eIF-4F 25kD Subunit, EIF4EL1, EIF4F)
Größe
£696.00
- SKU:
- E8949-40M
- Reinheit:
- Purified by ammonium sulfate precipitation
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide selected from the N-terminal region of human EIF4E (KLH).
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide.
- Weitere Details:
- eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. this gene also interacts mutually exclusive with EIF4A1 and EIF4A2. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:250-1:500 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice