E8947-95

EIF1, Recombinant, Human (Eukaryotic Translation Initiation Factor 1, A121, EIF-1, EIF1A, ISO1, SUI1)

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£767.00

SKU:: E8947-95
Anwendung:: WB
Molekulargewicht:: 16.9
Reinheit:: ~90% by SDS-PAGE.
Lagerbedingungen:: 4°C/-20°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl buffer, pH8, 1mM DTT, 0.1M NaCl, 10% glycerol.
Weitere Details:: EIF1, also known as eukaryotic translation initiation factor 1, is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. This protein promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Recombinant human EIF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Endotoxin: ≤1EU/1ug (LAL). Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMSA IQNLHSFDPF ADASKGDDLL PAGTEDYIHI RIQQRNGRKT LTTVQGIADD YDKKKLVKAF KKKFACNGTV IEHPEYGEVI QLQGDQRKNI CQFLVEIGLA KDDQLKVHGF Molecular Weight: 16.9kD (150aa) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.