E3565-10Q1
Estrogen Receptor alpha (ER alpha, Estradiol receptor, NR3A1)
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£862.00
- SKU:
- E3565-10Q1
- Anwendung:
- WB
- Reinheit:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to Met1-Gln116 from human ERa/NR3A1 expressed in E. coli.
- Klon:
- 11n67
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- ERa is a 65-70kD member of the NR3 subfamily, nuclear hormone receptor family of proteins. It is widely expressed, and serves as a strong activator of estrogen-responsive genes. ERa is normally quiescent and bound to heat-shock proteins and immunophilins. Following b-estradiol binding, it becomes activated, either homodimerizes or heterodimerizes with ERb, and binds to DNA with multiple coactivators. Human ERa is 595aa in length. It contains a DNA binding region aa185-250, three NLSs aa256-260; 266-271; 299-303, a steroid-binding site aa351-543, a dimerization motif aa497-518, and an O-GlcNAc attachment around Thr575. Major phosphorylation sites exist at Tyr537, Ser167 and Ser118. Multiple splice forms exist. There is an 80kD isoform that shows a substitution (duplication) of aa412-517 for Asp411, a second isoform with a deletion of aa255-366, a third isoform with a deletion of aa152-412, and a fourth isoform that shows a Thr substitution for aa152-595. Human ERa is only 46aa identical to human ERb. Over aa1-116, human ERa shares 85aa identity with mouse ERa. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.05ug/ml using MCF‑7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line under reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice