Estrogen Receptor alpha (ER alpha, Estradiol receptor, NR3A1)

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£895.00

SKU:: E3565-10K1
Anwendung:: WB
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Met1-Gln116 from human Estrogen receptor alpha, expressed in E. coli. Species Sequence Homology: mouse
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Weitere Details:: ER alpha (Estrogen receptor alpha; also Estradiol receptor and NR3A1) is a 65-70kD member of the NR3 subfamily, nuclear hormone receptor family of proteins. It is widely expressed, and serves as a strong activator of estrogen-responsive genes. ER alpha is normally quiescent and bound to heat-shock proteins and immunophilins. Following beta-estradiol binding, it becomes activated, either homodimerizes or heterodimerizes with ER beta, and binds to DNA with multiple coactivators. Human ER alpha is 595aa in length, and contains a DNA binding region (aa185-250), three NLSs (aa256-260; 266-271; 299-303), a steroid-binding site (aa351- 543), a dimerization motif (aa497-518), and an O-GlcNAc attachment around Thr575. Major phosphorylation sites exist at Tyr537, Ser167 and Ser118. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml detects MCF-7 human breast cancer cell line and MDA-MB-468 human breast cancer cell line under reducing conditions. Immunohistochemistry: 5-15ug/ml with immersion fixed paraffin-embedded sections of human endometrial cancer tissue. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice