E3405-50-ML650
Epithelial Cell Surface Antigen (ESA, Adenocarcinoma-associated Antigen, CD326, CD326 Antigen, Cell Surface Glycoprotein Trop1, Cell Surface Glycoprotein Trop-1, CO-17A, CO17-1A, Epithelial Cellular Adhesion Molecule, Ep CAM, EpCAM, Ep-CAM, Epithelial Glycoprotein, EGP, EGP40, GA733-2, hEGP-2, KS-1/4 Antigen, KSA, M1S2, M4S1, Major Gastrointestinal Tumor Associated Protein GA733-2, MIC18, MK-1, Tumor-associated Calcium Signal Transducer 1, TACD1, TACSTD1, TROP1) (MaxLight 650)
Größe
£940.00
- SKU:
- E3405-50-ML650
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- H69 cell line (small cell lung carcinoma).
- Klon:
- 10B1416 (VU-1D9)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
- Weitere Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. The epithelial cell adhesion molecule (Ep-CAM) is a 38kD cell surface antigen otherwise known as CD326, MOC31 and Ber-EP4. CD326 is a type 1 transmembrane glycoprotein. It is expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen and paraffin):1:200 for paraffin sections. Requires protein digestion pretreatment (e.g. trypsin, pronase) of paraffin sections. Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul Western Blot: Detects a band at ~38kD in HT-29 cell lysates. Optimal dilutions to be determined by the researcher. Positive Control (Immunohistochemistry): Large bowel Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice