E3375-06B
Epidermal Growth Factor Receptor (erbB, EGFR)
Größe
£934.00
- SKU:
- E3375-06B
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Extracellular domain of human recombinant EGFR protein. Cellular Localization: Cell membrane.
- Klon:
- 3F154
- Format:
- Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
- Weitere Details:
- EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1,-2,-3-4 or HER-1,-2,-3-4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 2-4ug/ml for 2hrs at RT Immunoprecipitation (Native): 2ug/mg protein lysate using Protein G Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires digestion with Protease XXV at 1mg/ml PBS for 5 minutes at 37°C. Optimal dilutions to be determined by the researcher. Positive Control: A431 cells. Skin, placenta or squamous cell carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice