EP4 Receptor

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£754.00

SKU:: E3364-41
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: A 16aa peptide within 2nd extracellular domain of human EP4 (1) coupled to KLH. An antibody made to this epitope has been shown to recognize 47 and 78kD EP4 bands in the kidney (2). Species sequence homology: monkey (100%); rabbit, sheep and canine (87%); mouse and rat (81%).
Format:: Supplied as a liquid in PBS, 0.1% BSA.
Weitere Details:: Prostanoids are the cyclooxygenase metabolites derived from C-20 unsaturated fatty acids (arachidonic acid) and include prostaglandin (PG) D2, PGE2, PGF2 alpha, PGI2, and thrombaxane (Tx) A2. The fatty acids precursors are released from the membrane phosphoplipids in response to various physiological and pathological stimuli by the action of phospholipase A2 and are converted to various prostanoids by the sequential actions of cyclooxygenases and the respective synthases. Prostaglandin PGE2 is one of the major prostaglandin produced during inflammation. A variety of PGE2-mediated effects on vascular smooth muscle tonus, glomerular cell function, renin release, and renal salt and water transport have been described. PGs also influence neuronal activity by modulating neurotransmitter release, sensitizing secretory fibers to noxious stimuli, or inducing fever and sleep. The actions of PGE2 are mediated by rhodopsin-type; G-protein coupled membrane receptors, termed E-prostanoid (EP) receptors or PTGERs. There are four subtypes of PGE receptors designated as EP1, EP2, EP3, and EP4 that are encoded by different genes and expressed differently in each tissue. The intracellular signaling also differs among the receptor subtypes. In general, EP receptors display a protein topology typical of GPCR - 7 TM domain, an extracellular N-terminus, and a large intracellular C-terminus. Applications: Suitable for use in ELISA. Other potential application, though not tested is Western Blot. Other applications not tested. Recommended Dilution:. ELISA: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Control Peptide: E3364-42 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice