E3362-01D-ML650
EphA4 Receptor (Ephrin A4 Receptor) (MaxLight 650)
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£991.00
- SKU:
- E3362-01D-ML650
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to the C-terminus of human EphA4 receptor protein.
- Klon:
- 6A70
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
- Weitere Details:
- MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. The Eph receptors are the largest family of receptor tyrosine kinases. They can be grouped into two subclasses based on sequence homology: the EphA and the EphB receptors. The ligands for the Eph receptors are the ephrins. The EphA receptors bind ephrin-A ligands, which are tethered to the membrane by a GPI linkage, and the EphB receptors bind ephrin-B ligands, which have a membrane spanning region. The Eph receptors are widely expressed during embryonic development, including in the nervous system and the vascular system. Ephrins and Eph receptors have been implicated in many developmental processes including neuronal network formation, guidance of cell migration, and axonal pathfinding. They remain expressed at lower levels in adult tissues and are upregulated in pathological conditions such as cancer. EphA4 is preferentially expressed in the embryonic and adult nervous system, including the adult hippocampus, and in platelets. EphA4 has previously been designated Cek8 in chicken, Tyro1 in rat, and Sek1 in mouse. Applications: Suitable for use in FLISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot (Human, mouse, chicken): 1-3ug/ml Immunoprecipitation (Human, mouse, chicken): 5ug Immunohistochemistry (Frozen, mouse): 3-10ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Western Blot, Immunoprecipitation: EphA4-transfected 293T cells and mouse brain lysates. Immunohistochemistry: EphA4-transfected 293T cells and mouse hippocampus. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice