E3330A
Eosinophil Major Basic Protein (Azide Free)
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£863.00
- SKU:
- E3330A
- Reinheit:
- Purified by Protein A affinity chromatography from tissue culture supernatant
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human eosinophil major basic protein (MBP)
- Klon:
- 1.B.787 (BMK13)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.1% BSA. No preservative added.
- Weitere Details:
- Eosinophil major basic protein is felt to play an important role in eosinophil function and perhaps endothelial injury in hypereosinophilic states. Proteoglycan 2, bone marrow, also known as PRG2, is a human gene. The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases. A 117-residue protein that predominates in eosinophil granules. It is a potent enzyme against helminths and is toxic towards bacteria and mammalian cells in vitro. The eosinophil major basic protein also causes the release of histamine from mast cells and basophils, activates neutrophils and alveolar macrophages, and is directly implicated in epithelial cell damage, exfoliation and bronchospasm in asthma. Structurally the major basic protein (MBP) is similar to lectins (sugar-binding proteins), and has a fold similar to that seen in C-type lectins. However, unlike other C-type lectins (those that bind various carbohydrates in the presence of calcium), MBP does not bind either calcium or any of the other carbohydrates that this family recognize. Instead, MBP recognizes heparan sulfate proteoglycans. The structure of MBP is available from the Protein Data Bank, accession number 1h8u[2] and 2brs[3]. Applications: Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Reacts with fresh frozen tissues that were sectioned (6um), dried for 1 hour at RT and fixed in 1:1 solution of methanol and acetone, and airdried for 1 hour. Sections can be stored wrapped in aluminium foil at -70°C until used. Immunohistochemistry (FFPE): 4% PFA (3hr) should be used for skin biopsies. 5uM sections are dewaxed, and rehydrated with water. A proteinase treatment of 0.1% trypsin for 15 minutes in water should be performed prior to blocking and antibody staining.The antibody reacts better in frozen tissues than in paraffin embedded tissues. Western Blot: Purified normal human eosinophils were treated with digitonin (5mg/ml) for 10 minutes before boiling in sample buffer and run on a 15% SDS-PAGE. Purified MBP migrates at ~14kD, and typical human samples range from 10-16kD. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice