E3226-01

Entactin (Nidogen)

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£659.00

SKU:: E3226-01
Reinheit:: Purified
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rat
Immunogen:: Partially purified preparation of laminin from EHS mouse tumor.
Klon:: 4A46
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Weitere Details:: Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Although the precise composition and structure of basal laminae varies from tissue to tissue, as well as within the same tissue at different developmental stages, most mature basal lamina contain type IV collagen, laminins, heparan sulfate proteoglycans and entactin (or nidogen). Entactin is a sulfated glycoprotein of ~150kD. Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (Frozen sections): 1:25-1:200 using avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Western Blot: 1:100-1:1000 Optimum dilutions to be determined by researcher. Hybridoma: X63 Ag 8.653 mouse myeloma cells with spleen cells from a Fisher rat. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.