E3222-78C
ENO2 (Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural Enolase, Neuron-specific Enolase, NSE)
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£704.00
- SKU:
- E3222-78C
- Reinheit:
- Purified by affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to amino acids 416-433, LGDEARFAGHNFRNPSVL.
- Klon:
- NSE-P1
- Format:
- Supplied as a liquid in PBS, 0.03% thimerosal.
- Weitere Details:
- Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers. Expression of these isozymes can be cell-type and development-specific. This gene encodes gamma enolase (enolase 2) and is expressed in mature neurons and cells of neuronal origin. The alpha/alpha homodimers are expressed in embryo and in most adult tissues. The alpha/beta heterodimers and the beta/beta homodimers are found in striated muscle, and the alpha/gamma heterodimers and the gamma/gamma homodimers are expressed in neurons. Neuron specific enolase is found in elevated concentrations in plasma and certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Applications: Suitable for use in Immunocytochemistry, Western Blot and Immunohistochemistry. ELISA has been reported in literature (ref. 2). Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin-fixed, paraffin-embedded): 5-10ug/ml Western Blot: 0.5-5ug/ml Immunocytochemistry: 5-10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice