E3222-75M

Enolase 1 (ENO1, 2-phospho-D-glycerate Hydro-lyase, alpha Enolase, C-myc Promoter-binding Protein, ENO1L1, MBP-1, MBPB1, MPB1, Non-neural Enolase, NNE, Phosphopyruvate Hydratase, PPH, Plasminogen-binding Protein) (Azide Free)

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£685.00

SKU:: E3222-75M
Reinheit:: Purified
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Full-length recombinant protein corresponding to aa1-435 of human ENO1, with a GST Tag. MW of GST Tag alone is 26kD, (P06733).
Klon:: 10C89
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added
Weitere Details:: Enolase 1 gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. Applications: Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilutions: Immunofluorescence: 10ug/ml using HeLa cell lysates Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.