EDG 7 (Endothelial Cell Differentiation Gene 7, edg-7, LPA3, Lysophosphatidic Acid Receptor 3)

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£672.00

SKU:: E2208-35B
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the N-terminal region of mouse LPA3.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Weitere Details:: Endothelial Cell Differentiation Gene-7, or EDG-7, encodes a 40kD protein belonging to a family of G-Protein Coupled Receptors (GPCR’s), which bind lysophospholipids. To date, 8 members of the EDG receptor family have been identified. These receptors are suggested to have a role in modulation of growth-related effects including cell proliferation and differentiation as well as protective, anti-apoptotic effects. EDG receptor expression is developmentally regulated and the expression of the 8 members of this family of proteins appears to be tissue specific. EDG receptors couple to G-proteins to signal through Ras and MAPK pathways and other protein tyrosine kinase pathways. EDG-7 is also known as LPA3. The ligand for EDG-7 is lysophosphatidic acid (LPA), containing an sn-2 unsaturated fatty acid ester. Ligand binding activates Gq. EDG-7 is expressed by prostate and other tissues. Lysophosphatidic acid receptor 3 (LPA3, EDG-7) is one of three lysophosphatidic acid (LPA) receptors (LPA1, LPA2, LPA3) that are members of large family of G-protein coupled receptors (GPCRs) that also include those for sphingosine-1-phosphate (S1P1-5) (1). The LPA receptors mediate many cellular responses including cytoskeletal rearrangements, cell proliferation, and inhibition of gap junction communication (2,3). Murine and human LPA3 have 353 amino acids with an estimated molecular weight of 40kD (4,5). The mRNA level of LPA3 is high in testes, kidney, and lung but low in intestine, heart, thymus, and stomach (5). Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2.5ug/ml Immunocytochemistry: 2.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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