E0255-55B

EBAG9, Recombinant, Human (Estrogen Receptor-binding Fragment-associated Gene 9 Protein, EB9, Cancer-associated Surface Antigen RCAS1, PDAF, Receptor-binding Cancer Antigen Expressed on SiSo Cells, RCAS1)

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£422.00

SKU:: E0255-55B
Reinheit:: ≥ 90% by SDS PAGE.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 20mM Tris pH8.0,1mM DTT, 2mM EDTA, 10% glycerol.
Weitere Details:: Estrogen receptor binding site associated antigen 9, also known as EBAG9, is a type III transmembrane protein that is predominantly located in the Golgi. This protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers, such as advanced breast and prostate cancers. As the EBAG9 acts to inhibit the growth of receptor-binding cells and induced apoptosis of immune cells, cancer cells might evade immune surveillance. So immunodetection of EBAG9 expression can be a negative prognostic indicator. Recombinant human EBAG9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant corresponding to aa28-213 of human EBAG9 (His-tagged) expressed in E. coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MRSGRGRKLS GDQITLPTTV DYSSVPKQTD VEEWTSWDED APTSVKIEGG NGNVATQQNS LEQLEPDYFK DMTPTIRKTQ KIVIKKREPL NFGIPDGSTG FSSRLAATQD LPFIHQSSEL GDLDTWQENT NAWEEEEDAA WQAEEVLRQQ KLADREKRAA EQQRKKMEKE AQRLMKKEQN KIGVKLS Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 3ug/ml 15% SDS-PAGE, ~23.4kD Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice