E0010-ML490
E2F1 Transcription Factor (E2F 1, Retinoblastoma associated protein 1, Retinoblastoma binding protein 3, PBR3, RBAP-1, RBBP3, RBP3) (MaxLight 490)
Größe
£1,023.00
- SKU:
- E0010-ML490
- Reinheit:
- Purified by Protein G affinity chromatography
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- GST fusion protein corresponding to full-length human E2F-1. Epitope: aa1-89 for clone KH20; aa342-386 for clone KH95.
- Klon:
- KH20 and KH95
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
- Weitere Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. E2F-1 was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry. Applications: Suitable for use in Western Blot, Immunoprecipitation and Electrophoretic Mobility Shift Assay. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml of antibody detected E2F-1 in a HeLa nuclear extract. Immunoprecipitation: 4ug immunoprecipitated E2F-1 from HeLa nuclear extract. Optimal dilutions to be determined by the researcher. Positive Antigen Control (Included): H1838: HeLa Nuclear Extract Hybridoma: SP2 mouse myeloma cells with immunized mouse splenocytes. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice