E0010-20-HRP
E2F1 Transcription Factor (E2F 1, Retinoblastoma associated protein 1, Retinoblastoma binding protein 3, PBR3, RBAP-1, RBBP3, RBP3) (HRP)
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£1,049.00
- SKU:
- E0010-20-HRP
- Reinheit:
- Purified by Protein A affinity chromatography from ascites.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant human E2F-1 protein.
- Klon:
- KH95
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).
- Weitere Details:
- E2F-1 is a transcription factor (437 amino acids, 46kD) that contains a cyclin A binding domain, a DNA binding domain, a dimerization domain, and a transactivation domain. E2F-1 is expressed in a wide variety of cell lines and tissues and is expressed broadly, but not uniformly, during mouse embryogenesis. E2F-1 binds almost exclusively to pRB and phosphorylation of E2F-1 may disrupt pRB/E2F complexes. Overexpression of E2F-1 or dE2F in normal cells causes high levels of apoptosis while p19 arf -deficient cells are partially resistant to E2F-1-induced apoptosis. p53-independent apoptosis requires the E2F-1 DNA-binding domain but not an intact transactivation domain. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice