D8065-34
DPP10 (Dipeptidyl peptidase 10, DPPY, DPRP3, DPL2) (Biotin)
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£863.00
- SKU:
- D8065-34
- Anwendung:
- WB
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Sf 21-derived recombinant human rhDPP10 extracellular domain isoform1.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
- Weitere Details:
- DPP10 (dipeptidyl peptidase 10; also DPPY, DPRP3 and DPL2) is a 91-97kD member of the peptidase S9B family. It is a type II transmembrane glycoprotein principally expressed in neurons and T cells. Although it is a peptidase member, it has no catalytic activity. Instead, it assists in the membrane trafficking and functioning of Kv4 K + channels. Human DPP10 is 796 amino acids (aa) in length. It contains a 34 aa cytoplasmic N-terminus and a 741 aa extracellular domain (ECD) (aa 56-796). Three potential isoforms exist, all involving aa substitutions limited to the first 20 amino acids. Produced in sheep immunized with purified, Sf 21-derived, recombinant human DPP10 isoform 1 extracellular domain (rhDPP10; aa 56-796; Accession # NP_065919, with a Pro340Ala substitution). Human DPP10 specific IgG was purified by human DPP10 affinity chromatography and then biotinylated. Applications: Suitable for use in Western blot. Other applications have not been tested. Recommended Dilutions: Western blot: 0. -0.2ug/ml with the The detection limit is ~2ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice