D4015-14

DnaK, aa385-546, Substrate Binding Domain, Recombinant, E. coli

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£691.00

SKU:: D4015-14
Molekulargewicht:: 17777
Reinheit:: ≥ 95% by SDS PAGE; Purified by using conventional chromatography techniques.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 2mM beta-mercaptoethanol and 1mM EDTA.
Weitere Details:: DNAK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli. Additional amino acid (Met) is attached at N- terminus. Molecular Weight: 17,777 (163 amino acids) Sequence: MDVKDVLLLD/ VTPLSLGIET/ MGGVMTTLIA/ KNTTIPTKHS/ QVFSTAEDNQ/ SAVTIHVLQG/ ERKRAADNKS/ LGQFNLDGIN/ PAPRGMPQIE/ VTFDIDADGI/ LHVSAKDKNS/ GKEQKITIKA/ SSGLNEDEIQ/ KMVRDAEANA/ EADRKFEELV/ QTRNQGDHLL/ HST Biological Activity: Not determined Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.