D3441-89A
DGKZ (Diacylglycerol Kinase zeta, DAG Kinase zeta, Diglyceride Kinase zeta, DGK-zeta, DAGK6)
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£863.00
- SKU:
- D3441-89A
- Anwendung:
- WB
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Goat
- Immunogen:
- Purified E. coli-derived, recombinant human DGK-Z (aa800-945).
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Weitere Details:
- DGK-Z (Diacylglycerol kinase zeta; also DAG kinase 6) is a 130kD member of the eukaryotic diacylglycerol (DAG) kinase family of proteins. It exists in both cytoplasm and nucleus, and is widely expressed, being found in neurons, skeletal muscle cells and endothelium. DGK-Z converts DAG into phosphatidic acid (PA). In the nucleus, this activates mTOR and promotes cell proliferation. In the cytoplasm, DGK-Z-generated PA activates PAK1, leading to increased cell motility. Human DGK-Z is 1117aa in length and contains two DAG-type Zn-finger regions (aa287-419), a MARCKS homology domain (aa448-462) and a catalytic domain (aa486-607). In addition to the 130kD protein, there are several splice variants that run about 114kD in SDS-PAGE. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice