D3165-02

11-dATP (Biotin) (Biotin-11-dATP)

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£798.00

SKU:: D3165-02
Molekulargewicht:: 882.71
Reinheit:: ≥95%
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 10mM Tris, pH 7.5. Labeled with Biotin.
Weitere Details:: Biotin-labeled Nucleotides are used for non-fluorescent labeling of nucleic acids in purification and detection applications. Biotin-11-dATP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the 7-Deaza position of adenine. Spectroscopic Properties: λmax 280nm; ε 12,700 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.