Custom Protein Expression, Mammalian

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SKU:: CS090-10
Hersteller:: United States Biological
Weitere Details:: Mammalian Expression: United States Biological offers a recombinant protein expression service in mammalian cells. Our high-level mammalian expression service allows you to scale up to 50 liters, request tag removal and is from a human cell line origin with full glycosylation Protein Expression Service in CHO-K1 or HEK293: Option 1 Transient Expression: Guaranteed delivery of 3mg or more protein if Phase I is successful. Estimated time: 5~6 weeks Phase I: * Cloning of your interest gene into vector with His-tag, or IgG-Fc tag. * Sequencing confirmation of insert. * Small scale (5ml) transfection to test the expression level and optimization. Estimated time: 3-4 weeks Phase II: * Large scale transfection (500ml) of recombinant vector into HEK293 and protein expression, evaluation, and optimization. * Protein purification from 500ml of culture using affinity column of Ni-NTA or Protein G. The yield ranks from 3 to 25mg per 500ml with an average of 5mg. Estimated time: 2 weeks [Optional: Tag-cleavage] Option 2 Stable Cell line Expression-Milestone Service: Phase I: * Cloning of your interest gene into vector with His-tag, or IgG-Fc tag, or other selected tag * Sequencing confirmation of insert * Transfection of recombinant vector into CHO-K1 * Generation and identification of cell expressing target protein Estimated time: 3 weeks Phase II Antibiotic Selection: * Cloning of expressing cell line, 2 cycles * Large-scale culture of stable cell line Estimated time: 5~8 weeks Phase III Purification: From 1 Liter of CHO-k1 culture using affinity column of Ni-NTA or Protein A. Estimated time: 2 to 3 weeks [Payment is due at the end of each phase]