C9097-19N
Cytokeratin 18 (K18, CK18, CYK18, KRT18)
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£504.00
- SKU:
- C9097-19N
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human breast carcinoma cell line PMC-42.
- Klon:
- DC-10
- Format:
- Supplied as a liquid in PBS. pH 7.4, 0.09% sodium azide.
- Weitere Details:
- Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40kD and 68kD. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins). Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml Immunohistochemistry (paraffin): 10ug/ml using human colon, skin Flow Cytometry: 1-4g/ml. Intracellular staining. Optimal dilutions to be determined by the researcher. Positive Controls: Immunocytochemistry MCF-7 human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line. ELISA: MCF-7 human breast adenocarcinoma cell line Matched Pairs: Detection: C9097-19N Capture: K0199-21A Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice