C7947-65B-FITC
CTAGE1 (Cutaneous T-cell Lymphoma-associated Antigen 1, Protein cTAGE-1, Cancer/Testis Antigen 21.1, CT21.1, CTAGE2, Cutaneous Lymphocyte Antigen, CLA) (FITC)
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£576.00
- SKU:
- C7947-65B-FITC
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rat
- Klon:
- HECA-452
- Format:
- Supplied as a liquid in PBS, pH 7.2, BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Weitere Details:
- Cutaneous Lymphocyte-assiciated Antigen (CLA) is a 140kD homodimer protein recognized by a unique monoclonal antibody. It is expressed on T lymphocytes in skin, subsets of peripheral blood memory T cells, NK cells, memory B cells and dendritic cells, as well as on monocytes, granulocytes and activated endothelial cells. CLA is a carbohydrate epitope of sialic acid- and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1), a surface glycoprotein expressed on majority of peripheral blood leukocytes. CLA is a subset ligand for E-selectin, P-selectin, and L-selectin. It plays a role in memory lymphocyte homing, tethering and rolling. Treatment of activated HUVEC cells with this antibody inhibits lymphocyte adhesion. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ul/10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice