C7910-28C

Creatine Kinase MM Isoenzyme III, Recombinant (CKMM) (Pichia)

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£970.00

SKU:: C7910-28C
Reinheit:: ~95% (SDS-PAGE). Purified under non-denaturing conditions.
Lagerbedingungen:: -70°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in TBS, 10mM beta-mercaptoethanol, pH 7.0-7.5, 0.09% sodium azide, 50% glycerol.
Weitere Details:: Creatine Kinase MM isoenzyme with identical amino acid sequence to that of the native enzyme. CK-MM is a 47kD dimeric protein comprised of 2 identical subunits. Purified in the enzymatically active form. Reacts with polyclonal antibodies to MM isoenzyme in ELISA. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Unit Definition: One unit will transfer one micromole of phosphate from creatine phosphate to ADP per minute at 37°C. Measured at 340nm as one equimolar amount of NADH produced by coupled reaction. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.