C7910-01

Creatine Kinase (CK, Creatine Phosphokinase, Creatine N-Phosphotransferase, CPK, Phosphocreatine Phosphokinase)

Größe

£607.00

SKU:: C7910-01
Reinheit:: Contaminants: ALKP: < 0.01%, GOT/AST: < 0.01%, GPT: < 0.01%, PK: 0.11%
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
CAS Number:: 9001-15-4
Format:: Supplied as a lyophilized powder.
Weitere Details:: Creatine kinase (CK) is widely distributed and seems to be primarily concerned with ATP regeneration. CK may represent 10-20% of muscle cytoplasmic protein. Protein: ≥ 0.6mg protein/mg (Coomassie) Activity: ≥ 250 U/mg protein (Dimension® Clinical Chemistry System) Specific Activity: ≥ 200U/mg protein Unit Definition: One Unit will transfer one micromole of phosphate from Creatine Phosphate to ADP per minute at 37°C. Measured at 340nm as one equimolar amount of NADH produced by a coupled reaction. Reconstitution: 1mg protein/ml in Tris buffered saline, pH 8.0 containing 1mM dithiothreitol and 1mM EDTA. Add glycerol to 50% (v/v) and store at -20°C. Further dilutions should be in the reconstitution buffer plus 1% BSA as a carrier. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute and aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Store dessicated.