C7854-02-HRP

Connexin 32 (CX32, C’B1, Gap Junction Beta1 Protein, Gap Junction Liver Protein) (HRP)

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£1,070.00

SKU:: C7854-02-HRP
Reinheit:: Purified IgG
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Directed against an epitope located between residues 95-125 in the central cytoplasmic loop of rat Cx32.
Klon:: 2Q252 (M12.13)
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Weitere Details:: Applications: Suitable for use in Immunohistochemistry (lightly formaldehyde fixed tissues) and Western Blot. Other applications have not been tested. Recommended Dilution: Western blot: Detects a 27kD Connexin 32 polypeptide in blots of rat hepatocyte gap junctions and an additional 47kD band corresponding to aggregates. Optimal dilution to be determined by the researcher. Hybridoma: Produced by the immunization of BALB/c mice with isdolated rat junctional complexes. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.