Complement C5, Human (Not for Export EU)

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£466.00

SKU:: C7850-24U1
Reinheit:: Serum
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Format:: Supplied as a lyophilized powder. No preservatives added. Reconstitute with 1ml sterile ddH2O.
Weitere Details:: C5 component of human complement is a plasma protein consisting of two polypeptide chains, a, b, bound by disulphide bonds. It has a molecular weight of 190kD. The activated protein forms with the complement factors C6-C9 the membrane attack complex. The average concentration in plasma is about 70ug/ml. Applications: Suitable for use in ELISA, Immunoprecipitation, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested. Recommended Dilutions: Immunoelectrophoresis: 2ul or equivalent against 120ul anti-serum . Double Radial Immunodiffusion (Ouchterlony): 10ul Use a rosette arrangement with 10ul in a 3mm diameter center well and 2ul serum samples (neat and serially diluted) in 2mm diameter peripheral wells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.