Complement C3, C3c Subunit (Not for Export EU)

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£466.00

SKU:: C7850-14S9
Reinheit:: Serum
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Pooled normal guinea pig serum
Format:: Supplied as a lyophilized powder. No preservatives added. Reconstitute with 1ml sterile ddH2O. Dilute further with PBS, pH 7.2.
Weitere Details:: C3 is the most abundant complement protein in guinea pig serum. Its biological function strongly resembles that of C3 in man and other laboratory animal species. It has a central role in the activation system being common in both pathways. Activation of C3 is achieved by specific limited proteolysis resulting in the increase of a number of degradation fragments. The anaphylatoxin C3a promotes smooth muscle contraction and increases vascular permeability; the large C3b fragment is involved in binding to the complement activator and can interact with specific receptors to allow efficient clearance of the activating cell or particle; degradation fragments of C3b (C3bi, C3c, C3dg and C3d) are important in receptor binding and clearance mechanisms, in virus neutralization and possible in the immune response. Applications: Suitable for use in IEP, Single and Double RID. Other applications have not been tested. Recommended Dilutions: IEP: Use 2ul serum, plasma or equivalent against 120ul antiserum. Double RID: Use a rosette arrangement with 10ul antiserum in a 3mm diameter center well and 2ul serum samples (neat and serially diluted) in 2mm diameter peripheral wells. SIngle RID: use 1% antiserum in the gel. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice