Complement C3b, Human (Not for Export EU)

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£607.00

SKU:: C7850-13U
Reinheit:: Serum
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: C3b isolated and purified from pooled normal human serum.
Format:: Supplied as a lyophilized powder. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Weitere Details:: C3b (C3c+C3d) has a molecular weight of 170,000. Applications: Suitable for use in ELISA, Immunoelectrophoresis and Radial Immunodiffusion. Other applications not tested. Recommended Dilutions: Immunoelectrophoresis: 120ul against 2ul human plasma or equivalent. Double Radial Immunodiffusion: Use a rosette arrangement with 10ul in 3mm diameter center well and 2ul plasma samples (neat and serially diluted) in 2mm diameter peripheral wells. Single Radial Immunodiffusion: 1% in gel. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.