C7510-66F
Collagen Type VII
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£850.00
- SKU:
- C7510-66F
- Reinheit:
- Ascites
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Insoluble fractions prepared from neonatal human foreskin epidermal cells.
- Klon:
- 8J1054 (LH7.2)
- Format:
- Supplied as a liquid, 15mM sodium azide.
- Weitere Details:
- Type VII collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa. Immunoelectron microscopy shows that all of the LH7.2 binding sites are localized at the inferior border of the lamina densa. No binding could be detected within the dermis underneath the basement membrane at the putative sites of anchoring plaques. The antibody reacts predominantly with the basement membrane zone of stratified squamous epithelia and ectodermally derived glands. No staining is present around "simple" glandular epithelia or around blood vessels, muscle and nerve fibers. Detailed staining patterns of antibody LH7.2 with Type VII collagen in various normal and malignant tissues have been described (1). There is no loss of staining by treatment of frozen tissue sections with enzymes such as bacterial collagenase, trypsin, pronase, neuraminidase, chondroitinase or hyaluronidase. Staining is lost by pepsin digestion and by fixation with methanol, and is reduced by formaldehyde and glutaraldehyde fixation. Applications: Suitable for use in Immunofluorescence, ELISA, Dot Blot, Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoelectron Microscopy. Other applications not tested. Recommended Dilutions: Immunofluorescence (Indirect): 1:1000 on human or other mammalian frozen sections Immunohistochemistry (Frozen): 1:2000 on human tongue section fixed in acetone. Specifically stains basement membranes. Optimal dilutions to be determined by the researcher. Cellular Localization: Secreted, extracellular space, extracellular matrix, basement membrane Hybridoma: Fusion of mouse myeloma cells and splenocytes from BALB/c mice Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice