C7510-53N

Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen Type IV Alpha 1, COL4A1, Collagen Alpha-2(IV) Chain, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, COL4A2, Collagen alpha-3(IV) Chain, Collagen Type IV Alpha 3, COL4A3, Collagen alpha-4(IV) Chain, Collagen Type IV Alpha 4, COL4A4, Collagen alpha-5(IV) Chain, Collagen Type IV Alpha 5, COL4A5, Collagen alpha-6(IV) Chain, Collagen Type IV Alpha 6, COL4A6, Arresten, ASLN, ATS, CA44, CA54, Canstatin, DKFZp686I14213, FLJ22259, Goodpasture Antigen, MGC42377, MGC167109)

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£876.00

SKU:: C7510-53N
Anwendung:: ICC
Reinheit:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Ser1441-Thr1669 from human Collagen IV a1, expressed in NS0 cells.
Klon:: 11n50
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Weitere Details:: COL4A1 (collagen IV a1) is a 185kD member of the type IV collagen family. It is a secreted glycoprotein that is expressed by multiple cell types, including fibroblasts, keratinocytes and endothelial cells. Two COL4A1 molecules interact with a 170kD a2 chain to form a collagen IV triple helix. This helix further interacts with other helices to generate covalent oligomers that form a scaffold in the basement membrane. Mature human COL4A1 is 1642aa in length. It has an N-terminal “7S” proregion aa28-172, a central collagenous domain that contains multiple Gly-based repeats aa173-1440, and a C-terminal domain that is proteolytically cleaved to generate a 25-28kD NC1 globular segment that has potent antiangiogenic activity aa1441-1669. Multiple splice forms exist. One shows a deletion of aa499-849, a second shows a seven aa substitution for aa513-1669, and a third shows a seven aa substitution for aa958-1669. Over aa1441-1669, human COL4A1 is 97% aa identical to mouse COL4A1. Applications: Suitable for use in Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 8-25ug/ml in immersion fixed T98G human glioblastoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice