Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen Of Basement Membrane Alpha 1 Chain, Collagen Of Basement Membrane Alpha 2 Chain, Collagen Type IV Alpha 1, Collagen Type IV Alpha 2, DKFZp686I14213, FLJ22259, Goodpasture antigen) (Biotin)

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£940.00

SKU:: C7510-52F
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Collagen Type IV from human and bovine placenta.
Format:: Supplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 10mg/ml BSA, 0.01% sodium azide. Labeled with Biotin. Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule..
Weitere Details:: Applications: Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:4000-1:8000. Assayed against 1ug of Collagen IV in a standard sandwich ELISA using S7973-97A: Streptavidin (HRP) and A0030: ABTS Stable Liquid Substrate 1.46mM/Liter for HRPO for 30 minutes at RT. Immunohistochemistry (Frozen tissue): 1:50-1:200 Western Blot: 1:5000-1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Certificate of Origin: All components used in the preparation of this product are derived from starting materals of North American origin. Collection was performed in USDA inspected facilities. All materilas have been inspected and certified to be free of disease. Suitable for export.

Versandbedingungen:: Blue Ice