Clostridium botulinum Neurotoxin Type C1 Heavy Chain (BoNT-C1)

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£863.00

SKU:: C5851-49B
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Asn866-Glu1291 from Botulinum Neurotoxin Type C1 Heavy Chain, expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Weitere Details:: BoNT-C1 (Botulinum Neurotoxin Type C1 [Heavy Chain]) is a 100kD C-terminal fragment of the C1 Clostridum botulinum neurotoxin (holo)precursor. It constitutes a nonenzymatic part of a Zn 2+ -dependent M27 family endoprotease that potently blocks neurosecretion. The inactive 1291aa, 150kD C1 precursor is cleaved between Lys449Thr450 to generate a heterodimeric toxin held together by a preexisting interchain disulfide bond between Cys437 and Cys453. The 100kD C-terminus (heavy chain) acts as a ligand for neuronal membrane ecto-acceptors, its inducing internalization with translocation. The 50kD N-terminal light chain acts as an intracellular peptidase that cleaves SNAP-25 and syntaxin. This is suggested to block noradrenalin release from a readily releasable pool of vesicles. Over aa866-1291, the C1 heavy chain shares less than 40% aa identity with the D-type neurotoxin heavy chain. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml with BoNT-C1 Heavy Chain Receptor Binding Domain Immunoprecipitation: 25ug/ml immunoprecipitates BoNT-C1 heavy chain spiked in E. coli cell lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice