C5839-65A
CLEC4D (C-type Lectin Domain Family 4, Member D, C-type Lectin Superfamily Member 8, C-type Lectin-like Receptor 6, CLEC-6, CLECSF8, MCL) (APC)
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£767.00
- SKU:
- C5839-65A
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gly52-Asn215 from human CLEC4D, expressed in NS0 cells. Species sequence homology: mouse (63%).
- Klon:
- 10K53
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Weitere Details:
- CLEC4D (C-type lectin domain family 4 member D), also known as CLCSF8, CLEC-6, and MCL, is a 30kD type II transmembrane (TM) glycoprotein that belongs to the CLR (C-type Lectin Receptor) family of molecules. It is synthesized as a 215aa protein that contains a 17aa N-terminal cytoplasmic domain, a 21aa TM segment, and a 177aa C-terminal extracellular region. The extracellular region shows a short stalk and a 118aa CRD (carbohydrate recognition domain). The nature of its carbohydrate ligand is unknown. CLEC4D is restricted to monocytes/macrophages and serves as an endocytic receptor. Homodimers and homotrimers form on the cell surface. The human CLEC4D extracellular region shares 63% aa sequence identity with the mouse extracellular region. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells in whole blood monocytes Optimal dilutions to be determined by the researcher. Hybridoma: Fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human CLEC4D. Flow Cytometry: 1. Whole blood monocytes cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking, add 10ul of C5839-65A to 1-2.5x10e5 cells and incubated for 30 minutes at RT. 3. Remove unbound antibody by washing the cells twice in F4568-10. Note that whole blood requires a RBC lysis step at this point. 4. Resuspend the cells in F4568-10 for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2B antibody. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Versandbedingungen:
- Blue Ice