C5067-71
Chondroitin Sulfate Proteoglycan (NG2, CSPG)
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£891.00
- SKU:
- C5067-71
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa1592-2222 of Chondroitin Sulfate Proteoglycan expressed as a truncated integral membrane form in HEK293 cells.
- Klon:
- 5H26 (132.38)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
- Weitere Details:
- Chondroitin sulfate proteoglycan 4 (UniProt: Q00657; also known as Chondroitin sulfate proteoglycan NG2, HSN tumor-specific antigen) is encoded by the Cspg4 (also known as Ng2) gene (Gene ID: 81651) in rat. Chondroitin sulfate proteoglycan NG2 is a single-pass type I membrane protein that plays a role in cell proliferation and migration and stimulates endothelial cells motility during microvascular morphogenesis. It is present in neural cells and in extraneural tissues, especially in the developing mesenchyme. Its level of expression is highest on immature, proliferating cells and decreases when these cells begin to differentiate. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells. It is a major chondroitin sulfate proteoglycan that is produced after spinal cord injury and is expressed by macrophages and oligodendrocyte progenitors. It is also reported to inhibit neurite outgrowth and growth cone collapse during axon regeneration. NG2 chondroitin sulfate proteoglycan is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off in the mature form. Applications: Suitable for use in Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml detected NG2 Chondroitin Sulfate Proteoglycan in Rat E16 brain tissue lysate. Immunofluorescence: 1:50 detected NG2 Chondroitin Sulfate Proteoglycan in rat brain frozen sections, post-fixed in ethanol. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-14C: IgG, Fc (HRP) Pab Gt x Mo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt x Mo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice