C5045-38
Cholesterol Acyltransferase 2 (Acyl Coenzyme A Cholesterol Acyltransferase 2, ACAT2, Sterol O-Acyltransferase 2)
Größe
£626.00
- SKU:
- C5045-38
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa3-20 (P3GGARLRLQRTEGLGGER20) of human ACAT-2
- Format:
- Supplied as a liquid in TBS, pH 7.4, 0.1% BSA, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- Acyl-Coenzyme A is cholesterol acyltransferase-2 (ACAT-2) which catalyzes the formation of cholesterol esters from cholesterol and long chain fatty acyl-coenzyme A. It is associated with lipoprotein particle secretion and thus plays an important role in the metabolism of triglyceride-rich lipoproteins like ApoB100.1,2,3 In humans, ACAT-2 is primarily expressed in the small intestine, whereas in other mammals, the enzyme is expressed in the liver and the small intestine.4,2 Human ACAT-2 has 522 amino acid with an estimated molecular weight of 60 kD.5 Detects ACAT-2 at about 60 kD in murine liver, intestine, and fibroblasts.6 Applications: Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunocytochemistry: 1:500, 1us/ml Immunohistochemistry: 1:500, 1ug/ml Western Blot: 1:250, 2ug/ml for 1 hour at RT. Overnight incubations at 4ºC with greater dilutions can produce optimal results. Optimal dilution to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice