C4275-35P
Cholera Toxin, beta (Cholera Enterotoxin beta, Choleragenoid, CTX B, CTXB, CTX-B, TOXB, VC1456) (FITC)
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£512.00
- SKU:
- C4275-35P
- Reinheit:
- Purified IgG fraction to a purity of >95%.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Purified Choleragenoid.
- Format:
- Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Weitere Details:
- Cholera toxin is comprised of two subunits, A and B. Subunit A is an ADP-ribosyltransferase, which disrupts the proper signaling of G proteins, leading to cell dehydration. The 12kD nontoxic subunit B ("choleragenoid"), which is assembled into a 60kD pentamer above pH 2, allows the protein complex to bind to cellular surfaces via the pentasaccharide chain of ganglioside GM1. Applications: Suitable for use in ELISA, Immunofluorescence, and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:10-1:50. Direct FA staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining. Optimal dilutions to be determined by the researcher. Titer: ELISA: >1:1000 Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice