C2585-25E
CD49f (CD49 antigen-like family member F, CD49f, DKFZp686J01244, FLJ18737, Integrin alpha-6, ITGA6B, VLA-6)
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£479.00
- SKU:
- C2585-25E
- Reinheit:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- UM-UC-9, human bladder cancer cells
- Klon:
- BQ16
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservative added.
- Weitere Details:
- Human CD49f molecules are integrin alpha-6 chains that are expressed as heterodimers with CD29 (beta1 integrin) or CD104 (beta4 integrin) and function as adhesion receptors. CD49f is expressed on platelets, monocytes, T lymphocytes and thymoctyes. The ligands for CD49f/CD29 include laminin and invasion. CD49f/CD29 has been shown to associate with CD63. Applications: Suitable for use in Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured Raji human tumor cells were washed and incubated 45 min on ice with 80ml of anti-CD49f antibody at 10mg/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.78 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice