C2575-01
cdk5 (Cyclin-dependent p31)
Größe
£793.00
- SKU:
- C2575-01
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human cdk5.
- Klon:
- 4i34 (DC17)
- Format:
- Supplied as a liquid in 0.1M Tris-glycine pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
- Weitere Details:
- Applications: Suitable for use in Western Blot, Immunocytochemistry, Immunoprecipitation and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml detects cdk5 in a RIPA lysate from non-stimulated human A431 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C2575-01 (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Previous lots of antibody detected cdk5 in RIPA lysates from mouse Wehi cells and rat PC12 cells. Immunoprecipitation: 4ug/500ug of A431 cell lysate. Immunocytochemistry: 5ug/ml stains cdk5 in human A431 cells fixed with 4% paraformaldehyde. Optimal dilutions to be determined by the researcher. Included Positive Antigen Control: A0001-50 A431 Cells, Non-Stimulated. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Recommended secondary antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt xMo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice