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C2549-50P

cdc2, p34

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£787.00

SKU:
C2549-50P
Reinheit:
Purified by Protein A affinity chromatography.
Lagerbedingungen:
-20°C
Hersteller:
United States Biological
Host:
Mouse
Immunogen:
Recombinant protein consisiting of the carboxy-terminal two-thirds of the Xenopus p34cdc2.
Klon:
3G92
Format:
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Weitere Details:
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Inhibition and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 0.1-1ug/ml Western Blot: 0.5-1ug/ml Immunoprecipitation: 5ug. Capable of immunoprecipitating cyclin protein associated with p34cdc2. Inhibition: Inhibits the activation of p34cdc2 kinase by cyclin proteins. Immunohistochemistry (Frozen): 5-10ug/ml. Optimal dilutions to be determined by the researcher. Positive Control: Western Blot: A431 and HeLa 3T3 cell lysates; Xenopus egg extracts. Immunohistochemistry: Tested on a variety of human tissues including tonsil (germinal center), spermatogonia and skin (basal layer). Epitope Location: The epitope core residues are thought to reside within amino acids 220-227 of the mouse p34cdc2 protein corresponding to the sequence LGTPNNEV. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Versandbedingungen:
Blue Ice