C2548-97D8-ML650
CD229 (Cell Surface Molecule Ly9, hLy9, Lymphocyte Antigen 9, Ly 9, Ly9, Ly-9, mLY9, SLAMF3, T Lymphocyte Surface Antigen Ly9 Precursor) (MaxLight 650)
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£1,081.00
- SKU:
- C2548-97D8-ML650
- Reinheit:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Mouse pre-B cell line 300.19 transfected with CD229 cDNA.
- Klon:
- 3H1998 (HLy9.1.25)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
- Weitere Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. CD229 is a member of the immunoglobulin superfamily and may have a function in cell signalling. The antigen is expressed at low levels by mature T and B lymphocytes and at higher levels by single-positive thymocytes. Expression at variable levels is also seen in some cell lines, such as Daudi, U937 and HUT78. Applications: Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: NS-1 myeloma cells with spleen cells from Balb/c mice. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice