C2548-84U1
CD154 (CD40 Ligand, Cd40l, CD40-L, Cd40lg, sCD40L, HIGM1, IGM, IMD3, Ly62, Ly-62, T-BAM, T-cell Antigen Gp39, TNF-related Activation Protein, TRAP, Tumor Necrosis Factor Ligand Superfamily Member 5, Tnfsf5) (FITC)
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£620.00
- SKU:
- C2548-84U1
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein G affinity chromatography from supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Hamster
- Immunogen:
- L cells transfected with CD40L
- Klon:
- 3H1900 (39H5)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with FITC.
- Weitere Details:
- CD154 was formerly known as CD40 ligand (CD40L). It is also known as gp39, a transmembrane glycoprotein. It is a type II integral membrane protein and a member of the tumor necrosis factor (TNF) family of ligands. CD154 is expressed by activated T-cells and serves an important function in T-APC cognate interaction through binding to CD40 on B cells, monocytes/macrophages and dendritic cells. CD154 is expressed by activated CD4+ve T lymphocytes. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: Neat. Use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Recommended Negative Controls: Hamster IgG Negative Control: FITC Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice